| 1 | 1 | Dan Ratner, Steph Ratner | 25-29 | 58:53 | Analyze |
| 2 | 1 | Tanner Gregurich, Shiloh Gregurich | 30-34 | 59:31 | Analyze |
| 3 | 2 | Brett Johnson, Lexi Johnson | 25-29 | 59:41 | Analyze |
| 4 | 2 | McCall Hoyt, Matthew Grundy | 30-34 | 59:53 | Analyze |
| 5 | 1 | Eric Bibb, Reese Littlewood | 16-24 | 1:00:00 | Analyze |
| 6 | 3 | Joseph Martello, Cassandra Gill | 30-34 | 1:00:05 | Analyze |
| 7 | 3 | Sean Fraser, Kaitlyn Colonna | 25-29 | 1:00:13 | Analyze |
| 8 | 4 | Kiefer Calisto, Kara VanDooijeweert | 30-34 | 1:00:29 | Analyze |
| 9 | 1 | Dustin Needham, Kim Stocklmeir | 40-44 | 1:00:38 | Analyze |
| 10 | 4 | Elizabeth Burds, Connor Weitz | 25-29 | 1:00:40 | Analyze |
| 11 | 5 | Nicholas Karpiuk, Lisa Rooke | 30-34 | 1:00:41 | Analyze |
| 12 | 5 | Juan Herrera, Vanessa Storaci | 25-29 | 1:00:48 | Analyze |
| 13 | 2 | Nick Digani, Liz Sheehan | 40-44 | 1:01:04 | Analyze |
| 14 | 6 | Artem Kapshuk, Rachael Bridgman | 30-34 | 1:01:12 | Analyze |
| 15 | 1 | Renee Swider, Justin Swider | 35-39 | 1:01:53 | Analyze |
| 16 | 7 | Matt Mason, Bella Stechschulte | 30-34 | 1:02:13 | Analyze |
| 17 | 8 | Anna Christensen, Alex Sroka | 30-34 | 1:02:23 | Analyze |
| 18 | 3 | Courtney France, Jason France | 40-44 | 1:02:45 | Analyze |
| 19 | 9 | Deana Jagielo, Anthony Jagielo | 30-34 | 1:02:48 | Analyze |
| 20 | 10 | Aaron Ordway, Sydney Hudson | 30-34 | 1:02:59 | Analyze |
| 22 | 7 | Brandon Orozco, Ashley Lira | 25-29 | 1:03:30 | Analyze |
| 23 | 2 | Lindsay Collins, Ethan Goldman | 35-39 | 1:03:47 | Analyze |
| 24 | 11 | Tricia Bolden, Ashley Stephen | 30-34 | 1:04:02 | Analyze |
| 25 | 4 | Alexander Valadja, Meghan Morris | 40-44 | 1:04:43 | Analyze |
| 26 | 1 | Sara Newton-Smith, David Kadio-Morokro | 45-49 | 1:04:50 | Analyze |
| 27 | 12 | CeCe Cronin, Dan Cronin | 30-34 | 1:04:51 | Analyze |
| 28 | 2 | James-Ryan Salvi, Lauren Hoffman | 16-24 | 1:04:52 | Analyze |
| 29 | 8 | Emily Nikolai, Tyler Finley | 25-29 | 1:04:58 | Analyze |
| 30 | 13 | Joshua Herrington, Kayla Christiansen | 30-34 | 1:05:05 | Analyze |
| 31 | 2 | Jeffrey Wolschlag, Krista Pufundt | 45-49 | 1:05:09 | Analyze |
| 32 | 14 | Dan Nides, Baylor McPherson | 30-34 | 1:05:29 | Analyze |
| 33 | 9 | Megan Stuart, Jack Hartigan | 25-29 | 1:05:31 | Analyze |
| 34 | 10 | Kayla Jordan, C.J Dick | 25-29 | 1:05:47 | Analyze |
| 35 | 11 | Lindsey Blanks, Samuel Humphries | 25-29 | 1:05:57 | Analyze |
| 36 | 12 | Joshua Peterson, Emma Diaz | 25-29 | 1:06:06 | Analyze |
| 37 | 15 | Sara Brumana, Andrea Zoccali | 30-34 | 1:06:11 | Analyze |
| 38 | 3 | Macy Corrona, Spencer Corrona | 16-24 | 1:06:25 | Analyze |
| 39 | 3 | Demetrios Mersinas, Sophia Deslauriers | 35-39 | 1:06:31 | Analyze |
| 40 | 4 | Noah Huyler, Keri Herman | 16-24 | 1:06:38 | Analyze |
| 41 | 13 | Jared Harrison, Trista Pringle | 25-29 | 1:06:45 | Analyze |
| 42 | 14 | Michael Goulard, Miranda Wurtz | 25-29 | 1:06:55 | Analyze |
| 43 | 5 | Allie Baker, Cobe Huizenga | 16-24 | 1:06:56 | Analyze |
| 44 | 16 | Madelaine Brown, Jordan Molzan | 30-34 | 1:07:03 | Analyze |
| 45 | 17 | Anna Willis, John Reynolds | 30-34 | 1:07:04 | Analyze |
| 46 | 18 | Avery Hohenstein, Rachel Schwab | 30-34 | 1:07:08 | Analyze |
| 47 | 19 | Danielle Berry, Trey Berry | 30-34 | 1:07:10 | Analyze |
| 48 | 4 | Kelly McClearn, Bryan Musolf | 35-39 | 1:07:15 | Analyze |
| 49 | 15 | Olivia Louthen, Joe Blanchet | 25-29 | 1:07:16 | Analyze |
| 50 | 6 | Addie Coppinger, Spencer Derthick | 16-24 | 1:07:16 | Analyze |
| 51 | 5 | Joshua Lambrecht, Kenzie Evans | 35-39 | 1:07:17 | Analyze |
| 52 | 16 | Mallory Harr, Jalen Washington | 25-29 | 1:07:29 | Analyze |
| 53 | 17 | Cody Walter, Lexi Lutheran | 25-29 | 1:07:30 | Analyze |
| 54 | 18 | Noah Strom, Madysen Strom | 25-29 | 1:07:31 | Analyze |
| 55 | 6 | yasmin tontat, andrew tarleton | 35-39 | 1:07:31 | Analyze |
| 56 | 20 | Haley Wallis, Jared Lincoln | 30-34 | 1:07:40 | Analyze |
| 57 | 21 | Christopher Meyers, Alexandra Meyers | 30-34 | 1:07:40 | Analyze |
| 58 | 5 | ADRIENNE CHEN-OK, Mykyta Yurtyn | 40-44 | 1:07:45 | Analyze |
| 59 | 6 | Michael Garcia, Nastia Mederos | 40-44 | 1:07:56 | Analyze |
| 60 | 7 | Derrick Brown, Laura Edwards | 35-39 | 1:08:08 | Analyze |
| 61 | 19 | Jayce Kuehnlein, McKenzie Smith | 25-29 | 1:08:16 | Analyze |
| 62 | 22 | Anna Baer, Kelly Gibbons | 30-34 | 1:08:20 | Analyze |
| 63 | 8 | Rosimayri Hernandez, Amaury Alvarez | 35-39 | 1:08:24 | Analyze |
| 64 | 20 | Amanda Arendt, Tristian Penick | 25-29 | 1:08:24 | Analyze |
| 65 | 23 | Ian Gaffney, Caroline Militello | 30-34 | 1:08:24 | Analyze |
| 66 | 7 | Harrison Cowden, Isabel Reisman | 16-24 | 1:08:38 | Analyze |
| 67 | 24 | Christopher Goins, Rebeca Ponce Ochoa | 30-34 | 1:08:43 | Analyze |
| 68 | 1 | David Miller, Lara Mele | 50-54 | 1:08:43 | Analyze |
| 69 | 25 | Ethan Mamuric, Emily Coleman | 30-34 | 1:08:44 | Analyze |
| 70 | 7 | Joe Miday, Sarina Petroni-Miday | 40-44 | 1:08:50 | Analyze |
| 71 | 9 | Sean Weckerly, Hannah Bare | 35-39 | 1:08:55 | Analyze |
| 72 | 10 | Franklin Mershon, Jessie Mershon | 35-39 | 1:08:59 | Analyze |
| 73 | 8 | Daniel Herman, Megan Herman | 40-44 | 1:09:01 | Analyze |
| 74 | 26 | Abigail Hoerchner, Tarail King | 30-34 | 1:09:07 | Analyze |
| 75 | 27 | Allison Shimer, Tatsuya Takizawa | 30-34 | 1:09:12 | Analyze |
| 76 | 28 | Luis Calderon, Leticia Garcia | 30-34 | 1:09:14 | Analyze |
| 77 | 29 | Eric Bridgewater, Heidi Bridgewater | 30-34 | 1:09:18 | Analyze |
| 78 | 21 | Kirsten Andersson, Ross Andersson | 25-29 | 1:09:20 | Analyze |
| 79 | 22 | Maddie Geiger, William Simpson | 25-29 | 1:09:21 | Analyze |
| 80 | 30 | Sean Arney, Kristen Arney | 30-34 | 1:09:22 | Analyze |
| 81 | 31 | Erin Crawford, Michael Crawford | 30-34 | 1:09:26 | Analyze |
| 82 | 11 | Ann Marie Lindsley, Rockefeller Smith | 35-39 | 1:09:29 | Analyze |
| 83 | 12 | Timothy Stanis, Sara White-Delehoy | 35-39 | 1:09:35 | Analyze |
| 84 | 23 | Anders Olsen, Maddy Kish | 25-29 | 1:09:35 | Analyze |
| 85 | 24 | Erin Tyler, Mike Tyler | 25-29 | 1:09:38 | Analyze |
| 86 | 9 | Michael Meeks, Jessica Cuddy | 40-44 | 1:09:42 | Analyze |
| 87 | 10 | Andrew McNelis, Katie Garcia | 40-44 | 1:09:43 | Analyze |
| 88 | 13 | Nathan Miller, Rachel Peterson | 35-39 | 1:09:45 | Analyze |
| 89 | 11 | Dustin Diefenderfer, Lindsey Diefenderfer | 40-44 | 1:09:45 | Analyze |
| 90 | 25 | Emma Stratton, Kyle Kempton | 25-29 | 1:09:48 | Analyze |
| 91 | 26 | Ryan Henning, Peyton Brueher | 25-29 | 1:09:54 | Analyze |
| 92 | 32 | Chloe Kinsel, Jered Sternaman | 30-34 | 1:09:55 | Analyze |
| 93 | 27 | Ruston Matherne, Kathryn Matherne | 25-29 | 1:09:56 | Analyze |
| 94 | 12 | Rhonda Henderson, Kenneth Henderson | 40-44 | 1:09:59 | Analyze |
| 95 | 33 | Lindsay Horstmann, Shaun Horstmann | 30-34 | 1:10:02 | Analyze |
| 96 | 13 | Evan Blankenship, Anina Blankenship | 40-44 | 1:10:03 | Analyze |
| 97 | 34 | Angely Jimenez, Nicholas Heyse | 30-34 | 1:10:06 | Analyze |
| 98 | 28 | Micah Wassink, Ryan Kim | 25-29 | 1:10:08 | Analyze |
| 99 | 14 | Kevin Lora, Mandi Lora | 35-39 | 1:10:14 | Analyze |
| 100 | 3 | Marcus Malcolm, Rebekah Greer | 45-49 | 1:10:19 | Analyze |
| 101 | 14 | Dana Thomas, Jacob Thomas | 40-44 | 1:10:20 | Analyze |