| 1 | 1 | Matt Duval, Melody Berrong, Amanda Sutton, Kevin Gregory | 40+ | 1:05:35 | Analyze |
| 2 | 1 | Andrew Garritson, Candice Brown, Hannah Tatro, Richard Pettus | U40 | 1:08:22 | Analyze |
| 3 | 2 | Andrew Lockhart, Ashlin Cosselman, Padowithz Alce, Courtney Roberts | U40 | 1:08:40 | Analyze |
| 4 | 3 | Raven McGregor, Monica Magcalas, Eric Roberson, Johnathan Carey | U40 | 1:09:42 | Analyze |
| 5 | 4 | Evy Lewis, Buddy McGrath, Rachel Trent, Kyle Ference | U40 | 1:11:34 | Analyze |
| 6 | 5 | Robert Calamas, Rico Tungol, Katie Page, Kiley Prevatte | U40 | 1:15:02 | Analyze |
| 7 | 6 | Nicholas Barnett, Robert Jefferson, Baylor Aycock, Christina Kiessling | U40 | 1:16:17 | Analyze |
| 8 | 7 | Shannen Smith, Kelly Dawsey, Indya Tatum, Antoine Smith | U40 | 1:16:32 | Analyze |
| 9 | 8 | Joshua Johnson, Megan Johnson, Luke Elliott, Mattie Johnson | U40 | 1:16:38 | Analyze |
| 10 | 9 | Ron Utreras, Julia Winecoff, Emma Howatt, Yasin Lee | U40 | 1:20:59 | Analyze |
| 11 | 10 | Kendra Fuentes, Vinny Orlando, Laila Orlando, Jack Mariana | U40 | 1:21:53 | Analyze |
| 12 | 11 | Kelly Nguyen, Luan Nguyen, Ashley Shaw, Dakota Hooper | U40 | 1:22:35 | Analyze |
| 13 | 2 | Tim Hanson, Erin Oglesby, Stacy Nabor, Chris Dunlap | 40+ | 1:22:53 | Analyze |
| 14 | 12 | Allison Fisher, Madison Pollak, Blake Ingram, Ryan Fraizer | U40 | 1:23:11 | Analyze |
| 15 | 13 | Courtney Tucker, Harry Buck, Kennedy Wilson, Malik Walker | U40 | 1:23:22 | Analyze |
| 16 | 3 | Willie Freeman, Sharee Downs, Brian Stewart, Terri Stewart | 40+ | 1:23:43 | Analyze |
| 17 | 4 | Cal Abel, Adrienne Hill, Bree Pattillo, Kris Funkhouser | 40+ | 1:23:45 | Analyze |
| 18 | 14 | Kate Lipa, Chase Howell, Ally Minson, Jordan Minniefield | U40 | 1:23:57 | Analyze |
| 19 | 15 | Eric Boone, Jerry Spence, Rebekah Langley, Whitney Spence | U40 | 1:25:56 | Analyze |
| 20 | 16 | Mark McCourry, Cheyenne Walsh, Fernanda Solano, Mateus Brito | U40 | 1:26:21 | Analyze |
| 21 | 17 | Missy Jones, Megan Robinson, Ethan Latimer, Dennis Owens | U40 | 1:28:04 | Analyze |
| 22 | 18 | Dariyen Naranjo, Angel Rodriguez, Maricela Garibay, Tracie Naranjo | U40 | 1:28:15 | Analyze |
| 23 | 19 | Montana Schug, Matt Green, Oksana Dimitrova, Isaac Jones | U40 | 1:28:37 | Analyze |
| 24 | 20 | Amish Brahmbhatt, Shirish Vadali, Ava Grace Sestan, Mackenzie Craig | U40 | 1:28:51 | Analyze |
| 25 | 21 | Taylor Bordelon, Alan Huerta, Edith Maldonado, Aliammar Malani | U40 | 1:29:37 | Analyze |
| 26 | 22 | Eli Jones, Liberty Hill, Fayth Lasala, Justus Hill | U40 | 1:29:43 | Analyze |
| 27 | 5 | Stephanie Kull, KEVIN MARCY, Kathy Cheek, Jimmy Nixon | 40+ | 1:31:36 | Analyze |
| 28 | 23 | Briana Blake, Jon Bartholomew, Carl Hood, Ana Pimentel | U40 | 1:34:16 | Analyze |
| 29 | 24 | Kim Hoang, Ayden Hoang, Chloe Carter, Kwakwu Kwarteng | U40 | 1:34:30 | Analyze |
| 30 | 25 | Brian Tinnell, Emma Nelson, Jace Nelson, Gracie Mogelnicki | U40 | 1:38:07 | Analyze |
| 31 | 26 | Darryl Haynes, Gabriel Murphy, LaReina Haynes, Pedro Malu | U40 | 1:39:24 | Analyze |
| 32 | 27 | Matthew Yancey, Jasmine Holland, Manny Moton, Janelle Moton | U40 | 1:41:37 | Analyze |
| 33 | 28 | Cesia Maldonado, Omar Neyazi, Mehek Jan, Matthew Muñoz | U40 | 1:47:15 | Analyze |
| 34 | 6 | Mercy Rosario, JAMES CIOFFI, Bert Griggs, Stephanie Griggs | 40+ | 1:58:27 | Analyze |